ENSmutants | Histology | Whole Mount Method

Whole Mount Staining of the ENS

diagram of step A
(A) Open the gut along the mesenteric border. Pin flat and fix tissues.

diagram of step A
(B) Seprate mucosa/submucosa from muscle layers of the gut. Stain for the ENS. View from the top surface.

Whole mount staining of the ENS allows enteric neurons and neuronal fibers to be more easily seen than with traditional sectioning methods.

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